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Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source BJM
Martínez-Trujillo,Aurora; Aranda,Juan S.; Gómez-Sánchez,Carlos; Trejo-Aguilar,Blanca; Aguilar-Osorio,Guillermo.
Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavipes; Pectinolytic enzymes; Constitutive enzymes; Inducible enzymes; Pectin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100006
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The effects of inorganic salts on biosynthesis of pectinolytic enzymes by Aspergillus Niger AgEcon
Mojsov, Kiro.
The paper examines effects of different inorganic salts in an apple pulp base on the production of pectinolytic enzymes with the aim of optimizing the medium for maximal enzyme production. The apple pulp combined with corn flour and simple mineral salts was used as a nourishing base in submerged production of pectinolytic enzymes by the fungus Aspergillus niger MK-15. The growth of the microorganism (dry weight) on different sources of nitrogen showed maximum dry weight with (NH4)2HPO4. The growth of the microorganism (dry weight) on different concentration of (NH4)2HPO4 (by 0.2% to 0.8%) provided maximal dry weight with 0.7% (NH4)2HPO4. The different inorganic salts (sources of nitrogen) on base stimulated the production of pectinolytic enzymes and...
Tipo: Journal Article Palavras-chave: Apple pulp; Fermentation; Inorganic salts; Pectinolytic enzymes; Aspergillus niger; Agribusiness; Research and Development/Tech Change/Emerging Technologies; L65.
Ano: 2010 URL: http://purl.umn.edu/94732
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